Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Supplier: EDVOTEK
300
EDVO300EA 274 EUR
EDVO300
Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase
Educational Materials Biology Educational Materials Biochemistry Learning Activities
When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

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