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You Searched For: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Product Image-X193-04

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor
Description: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

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Product Image-G193-04

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor
Description: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Product Image-M334-100ML

VWR® Lysis Buffer, Hypotonic

Catalog Number: (M334-100ML)
Supplier: VWR Chemicals
Description: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
UOM: 1 * 100 mL
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Caspase cell lysis buffer

Catalog Number: (ENZOBMLKI1170030)
Supplier: ENZO LIFE SCIENCES AG
Description: Caspase cell lysis buffer, Enzo Life Sciences
UOM: 1 * 30 mL

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Extraction reagents

Supplier: ENZO LIFE SCIENCES AG
Description: Concentrated lysis buffer for preparation of cell and tissue extracts.

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Product Image-TNB-4300-L100

RBC Lysis Buffer (10X)

Catalog Number: (TNB-4300-L100)
Supplier: Tonbo Biosciences
Description: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
UOM: 1 * 100 mL

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Image Unavailable-ENZOADI801496

Cell lysis buffer, RIPA

Catalog Number: (ENZOADI801496)
Supplier: ENZO LIFE SCIENCES AG
Description: The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
UOM: 1 * 100 mL

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Product Image-786-580

Gram Negative lysis buffer

Supplier: G-Biosciences
Description: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

Product Image-786-265

Research buffers and reagents

Supplier: G-Biosciences
Description: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Image Unavailable-ENZOADI801284

RIPA cell lysis buffer 2

Catalog Number: (ENZOADI801284)
Supplier: ENZO LIFE SCIENCES AG
Description: RIPA cell lysis buffer 2, Enzo Life Sciences
UOM: 1 * 100 mL

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HDAC cell lysis buffer

Catalog Number: (ENZOBMLKI3460020)
Supplier: ENZO LIFE SCIENCES AG
Description: Cell lysis buffer for Fluor de Lys HDAC assays
UOM: 1 * 20 mL

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Image Unavailable-ENZOADI801339

Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Catalog Number: (ENZOADI801339)
Supplier: ENZO LIFE SCIENCES AG
Description: Cell lysis buffer 4, Enzo Life Sciences
UOM: 1 * 100 mL

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Image Unavailable-AGLS400900

Cell lysis analysis kit, SideStep II

Catalog Number: (AGLS400900)
Supplier: AGILENT
Description: Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
UOM: 1 * 1 items

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Product Image-PIER89901

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Fisher Scientific
Description: The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Product Image-A4150.0500

Lysis buffer, CTAB

Supplier: PanReac AppliChem
Description: Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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