You Searched For: PCR Tests

Catalog Number: (SARTSMB95-1003)

Supplier: Sartorius

Description: The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

UOM: 1 * 1 items

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Supplier: BIOPREMIER

Description: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Supplier: PRIMERDESIGN

Description: PCR kits for the detection and simultaneous quantification of hepatitis viruses.

Supplier: PRIMERDESIGN

Description: PCR kits for the detection and simultaneous quantification of human herpes viruses.

Supplier: BIOPREMIER

Description: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Catalog Number: (BIOPGPCRSGLUTEN)

Supplier: BIOPREMIER

Description: Gluten detection test kit by using qPCR. Celiac disease is an autoimmune disorder associated with an intolerance to gluten proteins mainly present in wheat, barley, and rye.

UOM: 1 * 1 KIT

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Supplier: BIOPREMIER

Description: These kits allow the detection of allergic substances. Ideal for controlling and monitoring the various steps of food production.

Supplier: PRIMERDESIGN

Description: Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. Although more than 100 different human papillomavirus (HPV) types have been characterised HPV 6, HPV 11, HPV-16 and HPV 18 are the most clinically relevant.

Supplier: Sartorius

Description: The Microsart® RESEARCH Mycoplasma real-time PCR kit is especially designed for fast and reliable Mycoplasma contamination control in cell cultures, cell culture supernatants and media most applicable in research and development, e.g. biotech and biopharmaceutical research and development, universities and governmental research groups or for in-process control during biopharmaceutical production processes.

Supplier: Q-BIOANALYTIC GMBH

Description: Testing for pathogenic <i>E. coli</i> like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with <i>E. coli</i> strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic <i>E. coli</i>. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q-BIOANALYTIC GMBH

Description: Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: BIOPREMIER

Description: SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

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Supplier: PRIMERDESIGN

Description: PCR kits for the detection and simultaneous quantification of gastrointestinal pathogens.

Supplier: Q-BIOANALYTIC GMBH

Description: The Real-Time PCR screening kit is addressing three different targets: 35S promoter; NOS Terminator; CTP2-CP4 EPSPS. The tests are all in accordance with the JRC guideline. Advantage of the CTP2-CP4 EPSPS compared to FMV detection is that the used target includes artificial sequences, which are not present naturally like the virus sequence of FMV.

Supplier: Q-BIOANALYTIC GMBH

Description: Sus scrofa (pork) halal and kosher food does exclude pork meet. To make sure that pork meat is really absent in complex food it is advisable to test for the presence of it using our Real-Time test. Positive DNA and all required components are included in the kit.

Supplier: Q-BIOANALYTIC GMBH

Description: Cronobacter sakazakii (formerly Enterobacter sakazakii) is found naturally in the environment and can survive in dry conditions. It occurs in dry foods, such as powdered infant formula, milk powder and starches as well as in teas. Especially young infants are at high risk developing severe meningitis, if infected. All common Real-Time PCR block cyclers are usable for the test. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons. The test can also be used for the confirmation of bacterial colonies. The Real-Time PCR Test allows the detection of the species with high accuracy and is more precise than, cultural methods often are.