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You Searched For: Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-PIER90402

Mass tagging, mass tag labelling reagents, AminoxyTMT™, Pierce™

Supplier: Thermo Fisher Scientific
Description: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Product Image-PIER28340

Detergent sampler Kit, Surfact-Amps™

Catalog Number: (PIER28340)
Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
UOM: 1 * 1 KIT

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Product Image-PIER20230

Immobilised trypsin, TPCK treated, Pierce™

Catalog Number: (PIER20230)
Supplier: Thermo Fisher Scientific
Description: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM: 1 * 2 mL
Product Image-ICNA116978025

Lysing matrix Z, MP Biomedicals

Supplier: MP Biomedicals
Description: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

Product Image-PIER28336

Detergent solutions, Surfact-Amps™

Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

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Image Unavailable-324713-50

Endoproteinase Glu-C, excision grade, Staphylococcus aureus

Catalog Number: (324713-50)
Supplier: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 50 µG
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PopCulture® reagent

Supplier: Merck Millipore (Novagen)
Description: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Product Image-PIER90305

Trypsin protease, MS grade

Supplier: Thermo Fisher Scientific
Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Master mix, BugBuster®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Product Image-71009-4

Protein extraction reagents, CytoBuster™

Supplier: Merck Millipore (Novagen)
Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Image Unavailable-324708-2

Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Catalog Number: (324708-2)
Supplier: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 2 µG
Product Image-786-680

Washing solution, Protein-OUT™

Catalog Number: (786-680)
Supplier: G-Biosciences
Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL
Image Unavailable-VARIPL6008-4102

Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN
Description: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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