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You Searched For: Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Product Image-786-251

Albumin removal kit, AlbuminOUT™

Supplier: G-Biosciences
Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Catalog Number: (ENZOBMLKI1761250)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

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Product Image-PIER88700

Universal nuclease for cell lysis, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

MSDS

Product Image-786-276

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Image Unavailable-ENZOADI9502730500

Antigen retrieval reagent, IHC

Supplier: ENZO LIFE SCIENCES
Description: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Product Image-786-598

Biotin resin

Catalog Number: (786-598)
Supplier: G-Biosciences
Description: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM:


Product Image-786-249

Protein fractionation kit, FOCUS™ Membrane Proteins

Catalog Number: (786-249)
Supplier: G-Biosciences
Description: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-257

FOCUS™ Yeast proteome kits

Catalog Number: (786-257)
Supplier: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-244

SilverOUT™, G-Biosciences

Catalog Number: (786-244)
Supplier: G-Biosciences
Description: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 100 Tests
Product Image-786-793

Immobilised Ficin

Catalog Number: (786-793)
Supplier: G-Biosciences
Description: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
Product Image-DGA01

Optimizer blueBALLS™

Catalog Number: (DGA01)
Supplier: G-Biosciences
Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 items
Product Image-786-681

InGel™ Blue for protein digestion

Catalog Number: (786-681)
Supplier: G-Biosciences
Description: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 KIT
Product Image-PIER78260

Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Product Image-ENZOENZ450140100

EpiXtract® total histone extraction kit

Catalog Number: (ENZOENZ450140100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
UOM: 1 * 1 KIT

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Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certificates

Product Image-786-247

Protein fractionation kit, FOCUS™ soluble and insoluble

Catalog Number: (786-247)
Supplier: G-Biosciences
Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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