Trypan blue 0,4% in aqueous solution, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Danger
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Synonyms:
Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulphonic acid tetrasodium salt, CI 23850, 3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulphonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium salt, Benzamine blue, Trypan blue sodium salt, Direct Blue 14, Diphenyl Blue, AzidinBlue, Sodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulphonate)
M-Clarity™ quality level = MQ200
Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.
- it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents
- employed as a collagen stain in a Van Gieson procedure
- To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains
- used as a fluorescent tracer of cell populations in embryology
- used as a tumor promoter modulating permeability of lysosomal membranes
Physical form: 0,4% in 0,81% sodium chloride; 0,06% potassium phosphate, dibasic.
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Formula:
C₃₄H₂₄N₆Na₄O₁₄S₄ MW: 960,82 g/mol Density: 1,007 g/ml at 20 °C Storage Temperature: RT |
MDL Number:
MFCD00003969 CAS Number: 72-57-1 EINECS: 200-786-7 |
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Specification Test Results
| Appearance (colour) | Blue to very dark blue |
| Appearance (form) | Liquid |
| Thin layer chromatogr. | Corresponds to standard |
| Density d20/4 | 0.997 - 1.017 |
| Wavelength (1) (UV) | 600 - 610 nm |
| Extinction (1%/1 cm) (1) (UV) | |
| Solvent (UV) | Methanol |
| Expiry period | 30 months |
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