3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate

Supplier: Rockland Immunochemicals

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

TMBE-100 TMBM-100
ROCKTMBE-100EA 213 EUR
ROCKTMBE-100 ROCKTMBM-100
3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate
3,3',5,5'-Tetramethylbenzidine (TMB)

In the presence of peroxidases, TMB substrates can act as an electron donor for the conversion of peroxides to water.


  • Reaction can be halted with acid to change the TMB to yellow
  • Soluble blue end product read at 370 nm or 655 nm
  • Incubation time varies depending on the assay conditions


TMB ELISA peroxidase substrate (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate used for antibody reactivity in ELISA experiments.


TMB ELISA peroxidase substrate is designed for use in immunology and cancer research.


Delivery information: Supplied as a ready to use clear to very light blue colour liquid.

Formula: C₁₆H₂₀N₂
MW: 240,35 g/mol
MDL Number: MFCD00007748
CAS Number: 54827-17-7

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR