Protease and peptidase substrate

Supplier: Biotium

10202 10201 80103 10210 10205 10207 10208 10209 10211 10220 10225
10202.EA 391 EUR
10202. 10201. 80103. 10210. 10205. 10207. 10208. 10209. 10211. 10220. 10225.
Protease and peptidase substrate
Assays Enzyme Assays

Fluorogenic substrates for proteases and peptidases.


Rhodamine 110 and R110-PEG can be used as a reference standard for rhodamine 110-based peptidase substrates.


Z-FR-AMC is a substrate for serine proteases, including cathepsins, kallikrein and plasmin. Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.


BZAR is a substrate for serine proteases and is also an inhibitor for quanidinobenzoatase, 50 to 100 times more sensitive than the AMC-based substrate. BZiPAR is a substrate for trypsin, substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases.


Ac-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade. Z-FR-R110 is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.


D2R is a general substrate for caspases including caspases 3 and 7, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts. Z-AAD-R110 is a substrate for granzyme B. Granzyme B from T lymphocytes and natural killer cells induces apoptosis in target cells by activating caspases and causing mitochondrial cytochrome C release. Z-AA-R110-PEG is a substrate for elastate.

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