3,5-Dihydroxytoluene monohydrate (colorimetric), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Warning

Synonyms: 5-Methylresorcinol monohydrate, Orcinol monohydrate, 5-Methylbenzene-1,3-diol hydrate

M-Clarity™ quality level = MQ200

O1875-100G O1875-5G
O1875-100GEA 458 EUR
O1875-100G O1875-5G
3,5-Dihydroxytoluene monohydrate (colorimetric), Sigma-Aldrich®
3,5-Dihydroxytoluene monohydrate

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial's reagent for the determination of RNA, DNA, and hexoses.


Orcinol monohydrate has been used to prepare Bial's reagent for Bial's orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.

Formula: C₇H₈O₂·1H₂O
MW: 142,15 g/mol
Boiling Pt: 290 °C (1013 hPa) (lit.)
Melting Pt: 56…58 °C (lit.)
Density: 1,290 g/cm³ (4 °C)
Flash Pt: 159 °C
Storage Temperature: RT
MDL Number: MFCD00149092
CAS Number: 6153-39-5
EINECS: 207-984-2
Merck Index: 12,06995
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Specification Test Results

Appearance (Colour) White to Light Brown to Light Orange
Appearance (Form) Powder
Solubility (Colour) Colorless to Light Brown
Solubility (Turbidity)
200 mg + 4 ml H2O
Clear
Water
1H NMR Spectrum
10 - 15
Purity (HPLC) ≥99 %
Recommended Retest Period 3 years

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