3,5-Dihydroxytoluene monohydrate (colorimetric), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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Synonyms:
5-Methylresorcinol monohydrate, Orcinol monohydrate, 5-Methylbenzene-1,3-diol hydrate
M-Clarity™ quality level = MQ200
Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial's reagent for the determination of RNA, DNA, and hexoses.
Orcinol monohydrate has been used to prepare Bial's reagent for Bial's orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.
Formula:
C₇H₈O₂·1H₂O MW: 142,15 g/mol Boiling Pt: 290 °C (1013 hPa) (lit.) Melting Pt: 56…58 °C (lit.) Density: 1,290 g/cm³ (4 °C) Flash Pt: 159 °C Storage Temperature: RT |
MDL Number:
MFCD00149092 CAS Number: 6153-39-5 EINECS: 207-984-2 Merck Index: 12,06995 |
Specification Test Results
Appearance (Colour) | White to Light Brown to Light Orange |
Appearance (Form) | Powder |
Solubility (Colour) | Colorless to Light Brown |
Solubility (Turbidity) 200 mg + 4 ml H2O |
Clear |
Water 1H NMR Spectrum |
10 - 15 |
Purity (HPLC) | ≥99 % |
Recommended Retest Period | 3 years |
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