Crystal violet solution Grams for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
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Warning
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M-Clarity™ quality level = MQ200
Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.
- Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues
- It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyse meiotic structures
- It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears
The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionised dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.
Formula:
C₂₅H₃₀ClN₃ Density: 0,981 g/ml (20 °C) Flash Pt: 34 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD00011750 CAS Number: 548-62-9 UN: 1170 ADR: 3,II |
Specification Test Results
Appearance (colour) | Purple to very dark purple |
Appearance (form) | Liquid |
Thin layer chromatogr. | Corresponds to standard |
Density D20/4 | 0.970 - 0.985 |
Wavelength (1) (UV) | 585 - 595 nm |
Extinction (1%/1 cm) (1) (UV) | Report result |
Solvent (UV) | Water |
Expiry period | 36 months |
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