T4 polynucleotide kinase

Supplier: Thermo Fisher Scientific

EK0031 EK0032
EK0031EA 48.99 EUR
EK0031 EK0032
T4 polynucleotide kinase
Enzymes

Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).


  • Detection of DNA modification by the [³²P]-postlabelling assay
  • 5'-phosphorylation of oligonucleotides, PCR products, and DNA or RNA prior to ligation
  • Removal of 3'-phosphoryl groups
  • Phosphorylation of PCR primers


T4 polynucleotide kinase exhibits 5'-phosphatase activity and catalyses the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.


Labelling 5'-termini of nucleic acids to be used as probes for hybridisation, probes for transcript mapping, markers for gel electrophoresis, primers for DNA sequencing, primers for PCR.


Caution: For research use only. Not for use in diagnostic procedures.

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