DMNPE-caged Luciferin
Supplier: OZ BIOSCIENCES
DMNPE-caged luciferin is a bioluminescent luciferase substrate used to measure intracellular functions since it readily crosses cell membranes. It is a D-luciferin ester analogue of luciferin.
- High purity
- Cell permeable, efficient luciferin delivery into intact cells, even at neutral pH
- Ideal for in vivo and in vitro applications
- Induces prolonged release of luciferin by the action of cells esterases
- Allows to follow changes in gene expression in live cells
Once the caged luciferin is inside the cells, active luciferin can be released by the action of endogenous intracellular esterases or by a flash of UV light. This implies:
1. A more efficient delivery of luciferin into intact living cells and improved sensitivity analysis of in vivo luciferase assays.
2. A long-term measurement of luciferase activity since hydrolysis by esterases allows a continuous supply of active Luciferin.
DMNPE-caged luciferin is a good alternative to D-Luciferin classically used to measure luciferase activity in live cells. Indeed, the difficulty to deliver D-luciferin into living cells renders in vivo quantification limited to a certain extent - for example, permeability issues at neutral pH.
Suitable for whole animal imaging, reporter gene assays, ATP assays, pyrosequencing and luciferase fragment complementation.
Packaging: Packed in an amber bottle under nitrogen.
Formula:
C₂₁H₁₉N₃O₇S₂ |
CAS Number:
223920-67-0 |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...