Pierce™ Streptavidin, AP (Alkaline Phosphatase)

Supplier: Thermo Fisher Scientific

21323 21324
PIER21323EA 748 EUR
PIER21323 PIER21324
Pierce™ Streptavidin, AP (Alkaline Phosphatase)
Proteins and Peptides

Streptavidin, alkaline phosphatase (AP) conjugate includes streptavidin in a purified form, conjugated to alkaline phosphatase for substrate based detection.


Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. These products use a recombinant form of streptavidin having a mass of 53000 daltons and a near-neutral pI (6,8 - 7,5). Tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.


This enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including ELISA (0,1 µg/ml), Western blot (2 µg/ml), immunofluorescence (assay dependent), immunohistochemistry and fluorescence imaging.


Caution: For research use only. Not for use in diagnostic procedures.

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