Pierce™ Streptavidin, HRP (horseradish peroxidase)
Supplier: Thermo Fisher Scientific
Streptavidin, horseradish peroxidase (HRP) conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.
This enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting (2 µg/ml), ELISA (0,1 µg/ml), immunohistochemistry (1 to 10 µg/ml), and fluorescence imaging. Streptavidin, Horseradish Peroxidase conjugate is supplied as lyophilised powder in phosphate-based buffer for immediate reconstitution with water.
Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. These products use a recombinant form of streptavidin having a mass of 53000 daltons and a near-neutral pI (6,8 - 7,5). Tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
Caution: For research use only. Not for use in diagnostic procedures.
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