Safranine O solution for Gram staining, Sigma-Aldrich®
Supplier: Merck
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Warning
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M-Clarity™ quality level = MQ300
Counterstain for Gram staining
The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set. It is an IVD product and CE registered.
Formula:
C₂₀H₁₉ClN₄ Density: 0,98 g/cm3 (20 °C) Flash Pt: 49 °C Storage Temperature: Ambient |
MDL Number:
MFCD00011759 CAS Number: 477-73-6 |
Specification Test Results
Suitability for microscopy | passes test |
gram-positve microorganisms | blue-violet |
gram-negative microorganisms | pink to red |
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