Leishman's eosin-methylene blue modified solution stain for histology, Sigma-Aldrich®

Supplier: Merck

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Synonyms: Eosin and methylene blue Leishman, Eosin-Methylene blue dye Leishman, Leishman's stain, Leishman stain, Leishman's stain (eosin methylene blue compound)

1.05387.0500 1.05387.1022
1.05387.0500EA 51.6 EUR
1.05387.0500 1.05387.1022
Leishman's eosin-methylene blue modified solution stain for histology, Sigma-Aldrich®
Leishman's Eosin-Methylene blue

For staining of blood and bone marrow smears and clinical-cytological specimens


The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright's eosin methylene blue solution and May-Grünwald's eosin methylene blue solution, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). 500 mL of the IVD & CE-registered Leishman's eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells. It is registered as IVD and CE product.


Preparative capacity: 1000 ml


3200 slides automate/staining cell (diluted)
500 slides staining rack (undiluted)

Boiling Pt: 65 °C (1013 hPa)
Density: 0,79 g/cm3 (20 °C)
Flash Pt: 12 °C
Storage Temperature: Ambient
MDL Number: MFCD00131498
CAS Number: 12627-53-1
UN: 1230
ADR: 3 (6.1),II
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Specification Test Results

Suitability for microscopy (Blood smear) passes test
Erythrocytes reddish
Nuclei violet
Eosinophilic granules brick-red
Neutrophilic granules light violet
Lymphocyte cytoplasm Blue

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