Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

C9999-1000ML C9999-100ML
C9999-1000MLEA 212 EUR
C9999-1000ML C9999-100ML
Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®
Buffers Antibody Purification Buffers

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.


In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

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