Methylene Violet 3RAX 90% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride, 3-Amino-7-(diethylamino)-5-phenyl phenazinium chloride, N,N-Diethylphenosafranine, 3-Amino-7-(diethylamino)-5-phenylphenazinium chloride

M-Clarity™ quality level = MQ100

307505-1G 307505-5G
307505-1GEA 87 EUR
307505-1G 307505-5G
Methylene Violet 3RAX 90% (dye content), Sigma-Aldrich®
Methylene Violet 3RAX

Methylene Violet 3RAX has been used as a reagent in a preparation of citrate methylene violet, which is used for staining yeast cells. It is also used as an internal standard in tandem mass spectrometry (MS/MS) for the quantification of methylene blue in blood sample.


Methylene violet 3RAX [MV3RAX, 3-amino-7-(diethylamino)-5-phenyl phenazinium chloride] is a phenazinium dye and is cationic in nature. It has several applications in staining chemistry. Methylene violet 3RAX dye has an antimalarial property. It is used as a probe for investigating guest–host interactions in dendrimers and this may have relevance in drug–DNA interactions.

Formula: C₂₂H₂₃ClN₄
MW: 378,9 g/mol
Melting Pt: 285 °C (dec., lit.)
Storage Temperature: RT
MDL Number: MFCD00013433
CAS Number: 4569-86-2
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Specification Test Results

Appearance (Colour) Green to Very Dark Green
Appearance (Form) Crystalline Powder
Infrared Spectrum Conforms to Structure
Wavelength 231 - 237 nm
Extinction Coefficient ≥12000
Wavelength 271 - 277 nm
Purity (HPLC) Typically ≥90%

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