Janus green B 65% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY


M-Clarity™ quality level = MQ200

201677-25G 201677-5G
201677-25GEA 386 EUR
201677-25G 201677-5G
Janus green B 65% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®
Janus green B

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.


Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become coloured. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Formula: C₃₀H₃₁ClN₆
MW: 511,07 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00011758
CAS Number: 2869-83-2
EINECS: 220-695-6
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Specification Test Results

Appearance (Colour) Black, Very Dark Brown or VeryDark Green
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength, C=0.01 G/L 50% Ethanol 651 - 677 nm
Extinction Coefficient ≥20000
Wavelength 280 - 286 nm
Carbon ≥45.1%
Nitrogen ≥10.5%
Biological Stain Commision, Commission Certified Number: Pass
Dye Content ≥65%

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