Rose Bengal ≥80% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Sodium 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodo-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-bis(olate), Food Red No. 105, CI 45440, Acid red 94, Aniline Rose, Rose Bengal sodium

M-Clarity™ quality level = MQ100

198250-25G 198250-5G
198250-25GEA 201 EUR
198250-25G 198250-5G
Rose Bengal ≥80% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®
Rose Bengal

Rose bengal,certified by the Biological Stain Commission, Dye content ≥80 % (4,5,6,7-Tetrachloro-2',4',5',7'-tetraiodofluorescein disodium salt; Acid Red 94; Bengal Rose B sodium salt; Rose Bengal sodium salt).


  • Rose bengal has several microbilogical staining applications including:
  • Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
  • counterstaining hematoxylin
  • staining cellular inclusions, together with Bismark brown


Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C20H2O5I4Cl4Na2. It is an anionic water-soluble xanthene dye.

Formula: C₂₀H₂Cl₄I₄O₅Na₂
MW: 1017,63 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00151169
CAS Number: 632-69-9
EINECS: 211-183-3

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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength, C = 0.008 g/L; Water + 1 ml of 1% w/v Sodium Carbonate (Na₂CO₃) 546 - 550 nm
Extinction Coefficient ≥90000
Biological Stain Commission, Commission Certified Number: Pass
Dye Content ≥80%
Solubility (Turbidity), c = 1 mg/ml, H₂O Clear to Slightly Hazy
Solubility (Colour) Light Red to Dark Red and Pink

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