BS3 (Bis(sulphosuccinimidyl) suberate sodium salt) ≥93% (by NMR), Pierce™

Supplier: Thermo Fisher Scientific

Synonyms: bis(sulphosuccinimidyl)suberate, Suberic acid bis(3-sulpho-N-hydroxysuccinimide ester) sodium salt, BS³, Sulfo DSS

21580 21586
PIER21580EA 218 EUR
PIER21580 PIER21586
BS3 (Bis(sulphosuccinimidyl) suberate sodium salt) ≥93% (by NMR), Pierce™
BS3 (Bis(sulphosuccinimidyl) suberate sodium salt)

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.


  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Water-soluble; compare with DSS
  • Membrane-impermeable, allowing for cell surface labeling
  • High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates


BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.


Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ∼100 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₆H₁₈N₂Na₂O₁₄S₂
MW: 572,43 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00054982
CAS Number: 82436-77-9

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Specification Test Results

Description Pierce BS3 (Sulfo-DSS)
Product Type Crosslinker
Reactivity Sulfo-NHS Ester
Solubility Water
Product Line Pierce™
Form Powder

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