FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥95% (by HPLC), Ultrapure
Supplier: ENZO LIFE SCIENCES AG
Synonyms:
5-isothiocyanatofluorescein
, 5-FITC
, Fluorescein Isothiocyanate, Isomer I
, Fluorescein Isothiocyanate
, 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
Amine reactive dye
Fluorescein isothiocyanate (FITC) is an amine-reactive fluorescein derivative that yields conjugates with superior stability. Dye synthesis generally results in two isomers that differ slightly in protein binding and may elute differently under chromatographic conditions, complicating analysis in certain situations. The 5-isomer of FITC is the most widely used isoform and has a multitude of applications beyond protein peptide and nucleotide labeling. For example, it is commonly employed as a donor for FRET (Fluorescence Resonance Energy Transfer) based assay systems. An acceptor molecule, such as TRITC, is recommended in such FRET applications. Wavelength Maxima: Excitation 494 nm, Emission 520 nm.
Formula:
C₂₁H₁₁NO₅S MW: 389,381 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00005063 CAS Number: 3326-32-7 |
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