Bromocresol green sodium salt in ACS Reagent grade water ≥90% (by HPLC), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
Synonyms:
Bromocresol green water soluble, Sodium 2,6-dibromo-4-(3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-2,2-dioxido-3H-benzo[d][1,2]oxathiol-3-yl)-3-methylphenolate
M-Clarity™ quality level = MQ200
Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes colour from yellow to blue-green at pH 3,8 to 5,4. The monosodium salt of bromocresol green is highly ionisable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.
- Bromocresol green serves as a pH indicator in biology and medicinal experiments
- It is used widely as a vital stain to study blood-brain permeability
- It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes
- It is used in TLC for the visualisation of compounds with functional groups whose pKa is below 5
- It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity
- It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples
- It is suitable as a selective inhibitor in kidney physiology studies
- It is a diagnostic assay manufacturing, hematology, histology
Formula:
C₂₁H₁₃Br₄NaO₅S MW: 720 g/mol Melting Pt: 230 °C Storage Temperature: RT |
MDL Number:
MFCD00148898 CAS Number: 62625-32-5 EINECS: 263-657-4 |
Specification Test Results
Appearance (Form) | Powder or Crystals |
Infrared spectrum | Conforms to Structure |
Visual Interval 1, pH 3.8 = Yellow | Pass |
Visual Interval 2, pH 4.5 = Green | Pass |
Visual Interval 3, pH 5.4 = Blue | Pass |
Clarity of solution not more than a faint trace of turbidity or insoluble matter c = 0.1 g/100 ml; Water | Conforms |
Purity (HPLC) | ≥90 % |
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