Bromocresol green sodium salt in ACS Reagent grade water ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Bromocresol green water soluble, Sodium 2,6-dibromo-4-(3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-2,2-dioxido-3H-benzo[d][1,2]oxathiol-3-yl)-3-methylphenolate

M-Clarity™ quality level = MQ200

114367-25G 114367-5G
114367-25GEA 275 EUR
114367-25G 114367-5G
Bromocresol green sodium salt in ACS Reagent grade water ≥90% (by HPLC), Sigma-Aldrich®
Bromocresol green sodium salt

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes colour from yellow to blue-green at pH 3,8 to 5,4. The monosodium salt of bromocresol green is highly ionisable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.


  • Bromocresol green serves as a pH indicator in biology and medicinal experiments
  • It is used widely as a vital stain to study blood-brain permeability
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes
  • It is used in TLC for the visualisation of compounds with functional groups whose pKa is below 5
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples
  • It is suitable as a selective inhibitor in kidney physiology studies
  • It is a diagnostic assay manufacturing, hematology, histology

Formula: C₂₁H₁₃Br₄NaO₅S
MW: 720 g/mol
Melting Pt: 230 °C
Storage Temperature: RT
MDL Number: MFCD00148898
CAS Number: 62625-32-5
EINECS: 263-657-4
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Specification Test Results

Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Visual Interval 1, pH 3.8 = Yellow Pass
Visual Interval 2, pH 4.5 = Green Pass
Visual Interval 3, pH 5.4 = Blue Pass
Clarity of solution not more than a faint trace of turbidity or insoluble matter c = 0.1 g/100 ml; Water Conforms
Purity (HPLC) ≥90 %

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