Toluidine blue, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Toluidine blue O zinc chloride double salt

M-Clarity™ quality level = MQ200

89640-25G 89640-5G
89640-25GEA 186 EUR
89640-25G 89640-5G
Toluidine blue, Sigma-Aldrich®
Toluidine blue

Toluidine blue (C.l. 52040) is a diaminothiazine dye. It is also known as TBO, tolonium chloride, and toluidine blue O.


  • Toluidine blue is suitable for use in the following applications to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
  • In metachromatic staining of sulfated mucins and related materials
  • As staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone
  • Staining of mucins in water-miscible resins and of nucleic acids in epoxy resins


Toluidine blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulphates, and phosphate radicals. It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content. It is used as a diagnostic assay manufacturing, hematology, histology.

Formula: C₁₅H₁₆ClN₃S·0.5ZnCl₂
MW: 373,97 g/mol
Storage Temperature: RT
MDL Number: MFCD03548293
CAS Number: 6586-04-5
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Specification Test Results

Grade for microscopy (Hist., Vit.)
Quality level 200
Solubility Methanol: 0,01 g/10 ml, blue to very deep blue
εmax ≥800 at 621 - 636 nm in water

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