Gram′s safranin solution for Gram staining, for microscopy, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Safranin T solution

M-Clarity™ quality level = MQ100

94635-2.5L-F 94635-250ML-F
94635-2.5L-FEA 143 EUR
94635-2.5L-F 94635-250ML-F
Gram′s safranin solution for Gram staining, for microscopy, Sigma-Aldrich®
Gram′s safranin solution

Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.


  • Ready-to-use solution
  • Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems


Gram’s safranin solution has been used in the following applications:
as a non-toxic replacement for crystal violet for the quantification of biofilm formation
to develop a hyaluronic acid visualisation method
to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole

Formula: C₂₀H₁₉ClN₄
MW: 350,85 g/mol
Density: 0,986 g/ml at 20 °C
Flash Pt: 46 °C
Storage Temperature: RT
MDL Number: MFCD00011759
CAS Number: 477-73-6
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Specification Test Results

Appearance (colour) Red to very dark red
Appearance (form) Liquid
Thin layer chromatogr. Corresponds to standard
Density D20/4 0.984 - 0.988
Refractive index N20/D 1.338 - 1.340
Wavelength (1) (UV) 525 - 535 nm
Extinction (1%/1 cm) (1) (UV) Report result
Solvent (UV) Ethanol 50%
Expiry period 36 months

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