X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) enzyme substrate
Supplier: Thermo Fisher Scientific
Synonyms:
BCIG, X-GAL, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
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R0402EA
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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) enzyme substrate
X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate
Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies.
Formula:
C₁₄H₁₅BrClNO₆ MW: 408,63 g/mol Melting Pt: 237…239 °C Storage Temperature: Freezer |
MDL Number:
MFCD00005666 CAS Number: 7240-90-6 EINECS: 230-640-8 |
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