X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) enzyme substrate

Supplier: Thermo Fisher Scientific

Synonyms: BCIG, X-GAL, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol

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R0402 R0404
X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) enzyme substrate
X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate


Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies.

Formula: C₁₄H₁₅BrClNO₆
MW: 408,63 g/mol
Melting Pt: 237…239 °C
Storage Temperature: Freezer
MDL Number: MFCD00005666
CAS Number: 7240-90-6
EINECS: 230-640-8

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