Coomassie® brilliant blue R-250, pure, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Serva Blue R, Brillant blue R, N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulphophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulpho-benzenemethanaminium inner salt, sodium salt (1:1), Coomassie Blue R 250, Brilliant Cyanine 6B, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, Acid Blue 83, Brilliant Blue R250, Brilliant Blue R, Brilliant indocyanine 6B

M-Clarity™ quality level = MQ200

B7920-10G B7920-50G
B7920-10GEA 119 EUR
B7920-10G B7920-50G
Coomassie® brilliant blue R-250, pure, Sigma-Aldrich®
Coomassie® Brilliant Blue R-250

Brilliant Blue R has been used in staining of gels after electrophoresis. It has also been used to determine protein in the protein bodies. For detection of protein bands following electrophoresis.


  • Brilliant Blue R is a methanol based stain
  • It can also stain agarose gels
  • It consists of methanol and acetic acid

Formula: C₄₅H₄₄N₃NaO₇S₂
MW: 825,98 g/mol
Melting Pt: 174…180 °C
Storage Temperature: RT
MDL Number: MFCD00041762
CAS Number: 6104-59-2
EINECS: 228-060-5
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Lambda Max
in EtOH
586 - 592 nm
Solubility (Colour)
1 mg/ml H₂O
Dark Blue
Purity (HPLC) ≥ 70 %

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