Coomassie® brilliant blue R-250 ~50% (dye content), Technical Grade, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Serva Blue R, Brillant blue R, N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulphophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulpho-benzenemethanaminium inner salt, sodium salt (1:1), Coomassie Blue R 250, Brilliant Cyanine 6B, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, Acid Blue 83, Brilliant Blue R250, Brilliant Blue R, Brilliant indocyanine 6B

M-Clarity™ quality level = MQ200

B0149-100G B0149-25G B0149-500G B0149-5G
B0149-100GEA 260 EUR
B0149-100G B0149-25G B0149-500G B0149-5G
Coomassie® brilliant blue R-250 ~50% (dye content), Technical Grade, Sigma-Aldrich®
Coomassie® Brilliant Blue R-250

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).


  • Its other applications include:
  • as a protein stain on epoxy-embedded tissue sections
  • for quantitative micro densitometry of protein in sections
  • for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes
  • For detection of protein bands following electrophoresis

Formula: C₄₅H₄₄N₃NaO₇S₂
MW: 825,98 g/mol
Melting Pt: 174…180 °C
Storage Temperature: RT
MDL Number: MFCD00041762
CAS Number: 6104-59-2
EINECS: 228-060-5
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Specification Test Results

Appearance (Form) Powder
Suitability For Detection of Protein Bands Following Electrophoresis Suitable
Solubility (Colour) Very Dark Purple to Very Dark Blue and Dark Blue-Black
Carbon 32.0 - 42.0 %
Nitrogen 2.5 - 3.5 %
Dye Content Approximately 50%

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