Immobilon™ Block Noise Canceling Reagents
Supplier: Merck
Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.
- Ready-to-use
- Protein-free, reducing nonspecific binding, especially when using phosphoantibodies or avidin-biotin systems
- Suitable for the dilution of primary and secondary antibodies
- Sufficient for 19 miniblots (each 8×7 cm blot requires at least 26 ml)
- Compatible with streptavidin antibodies
- Storage: 20 to 25 °C
Bløk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques. Minimise background in western blots, increasing sensitivity for superior detection of even low-abundance proteins. Compatible with PVDF (Immobilon™ P, Immobilon FL) and nitrocellulose.
Block-CH reagent for HRP chemiluminescence detection
Block-FL reagent for fluorescence detection
Block-PO reagent for phosphoprotein detection via chemiluminescence or fluorescence
For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.
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