Immobilon™ Block Noise Canceling Reagents

Supplier: Merck

WBAVDP001 WBAVDCH01 WBAVDFL01
MILFWBAVDP001EA 202 EUR
MILFWBAVDP001 MILFWBAVDCH01 MILFWBAVDFL01
Immobilon™ Block Noise Canceling Reagents
Electrophoresis Reagents Western Blotting Reagents

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.


  • Ready-to-use
  • Protein-free, reducing nonspecific binding, especially when using phosphoantibodies or avidin-biotin systems
  • Suitable for the dilution of primary and secondary antibodies
  • Sufficient for 19 miniblots (each 8×7 cm blot requires at least 26 ml)
  • Compatible with streptavidin antibodies
  • Storage: 20 to 25 °C


Bløk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques. Minimise background in western blots, increasing sensitivity for superior detection of even low-abundance proteins. Compatible with PVDF (Immobilon™ P, Immobilon FL) and nitrocellulose.


Block-CH reagent for HRP chemiluminescence detection
Block-FL reagent for fluorescence detection
Block-PO reagent for phosphoprotein detection via chemiluminescence or fluorescence


For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

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