Xylenol orange tetrasodium salt in ACS Reagent grade water, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: Xylenol orange water soluble, 2,2',2'',2'''-((((1,1-Dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetic acid, sodium salt (1:4)

M-Clarity™ quality level = MQ200

398187-1G 398187-25G 398187-5G
398187-1GEA 69 EUR
398187-1G 398187-25G 398187-5G
Xylenol orange tetrasodium salt in ACS Reagent grade water, Sigma-Aldrich®
Xylenol orange tetrasodium salt

Xylenol orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.


  • Xylenol Orange has been used as a vital stain to study calcification in bone development, injury, and repair
  • It has been used in the fluorescent histochemical detection of alkaline phosphatase
  • It has been used in FBX (ferrous benzoic xylenol orange) gel preparation to study the effects of MRI (magnetic resonance imaging) parameters on image quality

Formula: C₃₁H₂₈N₂Na₄O₁₃S
MW: 760,59 g/mol
Melting Pt: 195 °C (dec., lit.)
Storage Temperature: RT
MDL Number: MFCD00044293
CAS Number: 3618-43-7
EINECS: 222-805-8
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Specification Test Results

Appearance (Colour) Very Dark Brown to Very Dark Red
Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Use Test Pass
Appearance (Clarity), Clarity of Solution Pass
Meets ACS Requirements Current ACS Specification
Recommended Retest Period 3 Years

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