O-Dianisidine dihydrochloride ≥95%, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Danger

Synonyms: 3,3'-Dimethoxybenzidine dihydrochloride

M-Clarity™ quality level = MQ200

D3252-100G D3252-25G D3252-5G
D3252-100GEA 1250 EUR
D3252-100G D3252-25G D3252-5G
O-Dianisidine dihydrochloride ≥95%, Sigma-Aldrich®
o-Dianisidine dihydrochloride

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.


  • Physical form: Crystalline


o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.


Caution: Probably carcinogenic.

Formula: C₁₄H₁₈Cl₂N₂O₂
MW: 317,21 g/mol
Melting Pt: 268 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00012488
CAS Number: 20325-40-0
EINECS: 243-737-5
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Specification Test Results

Appearance (Colour) White to Light Purple and Faint Red to Light Red and Faint Beige to Light Beige and Faint Grey to Light Grey and Pink
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Solubility (Turbidity)
25 mg/ml, H₂O
Clear to Very Slightly Hazy
Solubility (Colour) Colorless to Faint Yellow and Colorless to Light Red
Water (by Karl Fischer)
Carbon (anhydrous) 50.1 - 55.7%
Nitrogen (anhydrous) 8.3 - 9.3%
Titration by AgNO₃ (anhydrous)
% C
21.1 - 23.5%
Recommended Retest Period 4 Years

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