O-Dianisidine dihydrochloride ≥95%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
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Danger
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M-Clarity™ quality level = MQ200
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
- Physical form: Crystalline
o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.
Caution: Probably carcinogenic.
Formula:
C₁₄H₁₈Cl₂N₂O₂ Melting Pt: 268 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00012488 CAS Number: 20325-40-0 EINECS: 243-737-5 |
Specification Test Results
Appearance (Colour) | White to Light Purple and Faint Red to Light Red and Faint Beige to Light Beige and Faint Grey to Light Grey and Pink |
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Solubility (Turbidity) 25 mg/ml, H₂O |
Clear to Very Slightly Hazy |
Solubility (Colour) | Colorless to Faint Yellow and Colorless to Light Red |
Water (by Karl Fischer) | |
Carbon (anhydrous) | 50.1 - 55.7% |
Nitrogen (anhydrous) | 8.3 - 9.3% |
Titration by AgNO₃ (anhydrous) % C |
21.1 - 23.5% |
Recommended Retest Period | 4 Years |
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