Fast black K salt, Practical grade, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

F7253-100G F7253-25G F7253-50G
F7253-100GEA 200 EUR
F7253-100G F7253-25G F7253-50G
Fast black K salt, Practical grade, Sigma-Aldrich®
Fast black K salt

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.


  • Fast black K salt has various applications as listed below
  • To stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
  • As a visualisation agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
  • For the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE staining endopeptidase and leucine aminopeptidase activity


Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Formula: C₂₈H₂₄Cl₄N₁₀O₈Zn
Melting Pt: 150 °C
Storage Temperature: RT
MDL Number: MFCD00078471
CAS Number: 64071-86-9
EINECS: 264-656-1
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Specification Test Results

Appearance (colour) Brown or red
Appearance (form) Powder
Solubility (colour) 10 mg/ml water
Solubility (method) 10 mg/ml water
Carbon content 9.9 - 18.3 %
Nitrogen content 4.1 - 7.6 %
Dye content ≥ 25 %

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