Acridine orange (chlorid salt), hydrate ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

318337-1G 318337-5G 318337-250MG
318337-1GEA 127 EUR
318337-1G 318337-5G 318337-250MG
Acridine orange (chlorid salt), hydrate ≥98% (by HPLC), Sigma-Aldrich®
Acridine orange (chlorid salt)

Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.


  • A rigorously purified zinc-free material suitable for use in flow cytometry
  • Acridine Orange hydrochloride hydrate dye has been used to stain intracellular acidic vesicles
  • to measure lysosomal membrane permeability (LMP) or autophagy detection
  • Fluorescent stain for nucleic acids in agarose and polyacrylamide gels
  • Has been used to study apoptosis in various cell culture and tissue models

Formula: C₁₇H₂₀ClN₃·H₂O
Melting Pt: 284,..287 °C (lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00150070
CAS Number: 65-61-2
EINECS: 200-614-0
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wave Length, c = 0.005g/L; Water 489 - 495 nm
Extinction Coefficient Typically ≥14,000
Wave Length 227 - 233 nm
Carbon Typically 55.0% - 67.5%
Nitrogen Typically 11.0% - 14.0%
Purity (HPLC) ≥98.0 %
Zinc Typically <50ppm Zinc
Solubility (Turbidity) Clear
Solubility (Color), c = 0.1%, H2O Orange to Red-Orange
Water (by Karl Fischer) Typically <20% Water

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