Acridine orange (chlorid salt), hydrate ≥98% (by HPLC), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
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Warning
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M-Clarity™ quality level = MQ200
318337-1G
318337-5G
318337-250MG
318337-1GEA
127
EUR
318337-1G
318337-5G
318337-250MG
Acridine orange (chlorid salt), hydrate ≥98% (by HPLC), Sigma-Aldrich®
Acridine orange (chlorid salt)
Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.
- A rigorously purified zinc-free material suitable for use in flow cytometry
- Acridine Orange hydrochloride hydrate dye has been used to stain intracellular acidic vesicles
- to measure lysosomal membrane permeability (LMP) or autophagy detection
- Fluorescent stain for nucleic acids in agarose and polyacrylamide gels
- Has been used to study apoptosis in various cell culture and tissue models
Formula:
C₁₇H₂₀ClN₃·H₂O Melting Pt: 284,..287 °C (lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00150070 CAS Number: 65-61-2 EINECS: 200-614-0 |
Specification Test Results
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Wave Length, c = 0.005g/L; Water | 489 - 495 nm |
Extinction Coefficient | Typically ≥14,000 |
Wave Length | 227 - 233 nm |
Carbon | Typically 55.0% - 67.5% |
Nitrogen | Typically 11.0% - 14.0% |
Purity (HPLC) | ≥98.0 % |
Zinc | Typically <50ppm Zinc |
Solubility (Turbidity) | Clear |
Solubility (Color), c = 0.1%, H2O | Orange to Red-Orange |
Water (by Karl Fischer) | Typically <20% Water |
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