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Nitro blue tetrazolium chloride (NBT) ≥90% (by HPLC), Sigma-Aldrich®

Description: Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.
Catalog Number: N6876-5G
UOM: 1 * 5 g
Supplier: SIGMA ALDRICH MICROSCOPY
Product Image-786-383

femtoCHROMO™- AP Substrate for chromogenic detection

Description: A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
Catalog Number: 786-383
UOM: 1 * 200 mL
Supplier: G-Biosciences
Image Unavailable-10003.

BCIP/NBT kit

Description: This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).
Catalog Number: 10003.
UOM: 1 * 1 SET
Supplier: Biotium
Image Unavailable-ROCKNBT-100

BCIP/NBT alkaline phosphatase substrate

Description: BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Catalog Number: ROCKNBT-100
UOM: 1 * 100 mL
Supplier: Rockland Immunochemicals

Certificates


Image Unavailable-ROCKBCBT-100

BCIP/TNBT alkaline phosphatase substrate

Description: BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Catalog Number: ROCKBCBT-100
UOM: 1 * 100 mL
Supplier: Rockland Immunochemicals
Image Unavailable-MOLE66601772-5G

5-Bromo-2-thiophenesulphonyl chloride

Description: 5-Bromo-2-thiophenesulphonyl chloride
Catalog Number: MOLE66601772-5G
UOM: 1 * 5 g
Supplier: Molekula
Image Unavailable-ICNA0219399950

Nitro blue tetrazolium chloride (NBT) for molecular biology

Description: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Catalog Number: ICNA0219399950
UOM: 1 * 50 mg
Supplier: MP Biomedicals
Image Unavailable-8.02699.0050

(±)-α-Chlorophenylacetyl chloride

Description: (±)-α-Chlorophenylacetyl chloride
Catalog Number: 8.02699.0050
UOM: 1 * 50 mL
Supplier: Merck
Image Unavailable-8.14055.0025

2-Mesitylenesulphonyl chloride for synthesis

Description: 2-Mesitylenesulphonyl chloride for synthesis
Catalog Number: 8.14055.0025
UOM: 1 * 25 g
Supplier: Merck

MSDS


Image Unavailable-74202-250G-F

Tetramethylammonium chloride, Supelco®

Description: Tetramethylammonium chloride, Supelco®
Catalog Number: 74202-250G-F
UOM: 1 * 250 g
Supplier: Merck
Image Unavailable-1.19203.0025

Silver chloride, Supelco®

Description: Silver chloride, Supelco®
Catalog Number: 1.19203.0025
UOM: 1 * 25 g
Supplier: Merck

MSDS


Image Unavailable-60136-250ML

Potassium chloride, Supelco®

Description: Potassium chloride, Supelco®
Catalog Number: 60136-250ML
UOM: 1 * 250 mL
Supplier: Merck
Image Unavailable-8.00678.0050

Succinyl chloride, Sigma-Aldrich®

Description: Succinyl chloride, Sigma-Aldrich®
Catalog Number: 8.00678.0050
UOM: 1 * 50 mL
Supplier: Merck

MSDS


Image Unavailable-8.20103.0050

p-Anisidinium chloride, Sigma-Aldrich®

Description: p-Anisidinium chloride, Sigma-Aldrich®
Catalog Number: 8.20103.0050
UOM: 1 * 50 g
Supplier: Merck

MSDS


Image Unavailable-201715-5G

Pinacyanol chloride, Sigma-Aldrich®

Description: Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).
Catalog Number: 201715-5G
UOM: 1 * 5 g
Supplier: SIGMA ALDRICH MICROSCOPY
Image Unavailable-86852-50G-F

Tetrabutylammonium chloride, Supelco®

Description: Tetrabutylammonium chloride, Supelco®
Catalog Number: 86852-50G-F
UOM: 1 * 50 g
Supplier: Merck
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