You Searched For: Assay+Buffers

Supplier: VWR Chemicals

Description: Biological buffers are useful for cell culture in vitro, enzyme assays, and some electrophoretic applications at physiological pH.

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Catalog Number: (E854-100RXN)

Supplier: VWR Chemicals

Description: PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

UOM: 1 * 1 KIT

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Catalog Number: (E169-100G)

Supplier: VWR Chemicals

Description: Biological buffers are useful for cell culture <i>in vitro</i>, enzyme assays, and some electrophoretic applications at physiological pH.

UOM: 1 * 100 g

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Catalog Number: (ROCK712-1831)

Supplier: Rockland Immunochemicals

Description: Supplied in pH 7.6 buffer (10 mM Sodium Phosphate 250 mM NaCl) Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

UOM: 1 * 1 mL

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Catalog Number: (ENZOBMLAK0140001)

Supplier: ENZO LIFE SCIENCES

Description: Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300 nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50 mM HEPES, pH 7,0, 10 mM CaCl₂, 0,05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.

UOM: 1 * 1 KIT

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Supplier: VWR Chemicals

Description: A biological buffer used for cell culture, <i>in vitro</i>, enzyme assays and some electrophoretic applications at physiological pH.

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Catalog Number: (STMC01702)

Supplier: STEMCELL Technologies

Description: Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

UOM: 1 * 55 mL

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Supplier: Thermo Fisher Scientific

Description: Kinase buffer I (5X)

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Supplier: Biosensis

Description: The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Catalog Number: (J62982.AE)

Supplier: Thermo Fisher Scientific

Description: Phosphate-Citrate Buffer (5X) is used as a substrate buffer in immunoassay procedures. It is used in SDS PAGE electrophoresis.

UOM: 1 * 100 mL

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Supplier: Biotium

Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2 , Na , K , fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Supplier: Biotium

Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2 , Na , K , fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Supplier: Biotium

Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2 , Na , K , fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Supplier: Biotium

Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2 , Na , K , fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Catalog Number: (BNUM0398-50)

Supplier: Biotium

Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2 , Na , K , fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

UOM: 1 * 50 µl

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Supplier: Thermo Fisher Scientific

Description: Kinase buffer II (5X)

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