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Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Catalog Number: (EDVO255)
Supplier: EDVOTEK
Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Product Image-K0251

Maxima™ SYBR® green QPCR master mix (2X), with ROX

Supplier: Thermo Fisher Scientific
Description: The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Product Image-F-566L

Phusion® green hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS
Description: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Image Unavailable-732-3645

Caps for VWR® Roller Bottles

Catalog Number: (732-3645)
Supplier: VWR Collection
Description: Sterile vented cap indivually wrapped, green, for 2000 ml bottes
UOM: 1 * 24 items

Environmentally Preferable


Product Image-MOTI1101001900302

Filters, Panthera

Supplier: MOTIC
Description: Panthera, Green filter (Ø 45 mm)

Product Image-525-1179

VWR®, Flat Screw Caps for Microcentrifuge Tubes

Catalog Number: (525-1179)
Supplier: VWR Collection
Description: Flat screw cap with O-ring for microcentrifuge tubes, VWR®, Colour: Green
UOM: 1 * 500 items

Environmentally Preferable


Product Image-BRDY174321

Multi-Purpose Vinyl Labels for M610, M611, BMP61, M710 (with media adapter) and BMP71 (with media adapter)

Supplier: Brady
Description: Permanent vinyl (B-439) is a thermal transfer printable material ideal for applications needing various colours like finished product ID, rating plates, and general-purpose ID.

Product Image-631-1138

VWR®, Dispensers for Sealing Films

Catalog Number: (631-1138)
Supplier: VWR Collection
Description: ABS dispenser designed to store, dispense and cut Parafilm™, tape, labels and Tough-Tags™.
UOM: 1 * 1 items
Product Image-MFLX96130-44

Masterflex® Transfer Tubing, Peroxide-Cured PTFE, Green, Avantor®

Supplier: Avantor Fluid Handling
Description: Quickly identify lines.

Certificates

Image Unavailable-SARS65.716.726

Colour coded screw caps in special sterile packaging

Catalog Number: (SARS65.716.726)
Supplier: SARSTEDT
Description: Colour coded screw caps, green
UOM: 1 * 5.000 items

Market Source Item This is a MarketSource item. Additional charges may apply

Product Image-AATB101410U1

Anti-CD14 Mouse Monoclonal Antibody [clone: 61D3] (mFluor™ Green 620)

Supplier: AAT BIOQUEST
Description: 61D3 is an anti-human monoclonal antibody that targets the CD14 antigen.

Product Image-AATB101610U1

Anti-CD16a Mouse Monoclonal Antibody [clone: 3G8] (mFluor™ Green 620)

Supplier: AAT BIOQUEST
Description: The 3G8 monoclonal antibody recognises human CD16a, a 50 to 65 kDa transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils.

Product Image-AATB17646

Helixyte™ Green Fluorimetric dsDNA Quantitation Kits *Optimised for Broad Dynamic Range*

Supplier: AAT BIOQUEST
Description: DNA Quantitation is a very important task in DNA sample preparations for various analyses.

Product Image-AATB100520U1

Anti-CD5 Mouse Monoclonal Antibody [clone: UCHT2] (mFluor™ Green 620)

Supplier: AAT BIOQUEST
Description: UCHT2 is an anti-human monoclonal antibody that is specific for the CD5 antigen.

Product Image-AATB104410U0

Anti-CD44 Rat Monoclonal Antibody [clone: IM7] (mFluor™ Green 620)

Supplier: AAT BIOQUEST
Description: IM7 is an anti-human/mouse monoclonal antibody that targets the CD44 antigen.

Image Unavailable-ICNA0219502305

ABTS (Diammonium 2,2'-azinobis[3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate]), light green powder

Catalog Number: (ICNA0219502305)
Supplier: MP Biomedicals
Description: 2,2-Azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) has been used as a chromogenic substrate for horseradish peroxidase (HRP), both in general activity assays and in ELISA applications. Activity of HRP using ABTS appears about four-fold higher than using pyrogallol. It is mainly used as a substrate in sensitive peroxidase assays.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
UOM: 1 * 5 g
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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