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Product Image-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Catalog Number: (EDVO203)
Supplier: EDVOTEK
Description: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Product Image-EDVO110

Experimentation kits, molecular weight determination of proteins (agarose-based)

Catalog Number: (EDVO110)
Supplier: EDVOTEK
Description: This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.
UOM: 1 * 1 KIT
Product Image-EDVO153

Experimentation kits, determination of protein molecular weight (polyacrylamide-based)

Catalog Number: (EDVO153)
Supplier: EDVOTEK
Description: Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
UOM: 1 * 1 KIT

MSDS


Product Image-EDVO115-C

Kits: family pedigree cancer gene detection, Ready-to-Load™

Supplier: EDVOTEK
Description: In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Product Image-EDVO274

Experimentation kits, in search of the 'Kissing Disease'

Catalog Number: (EDVO274)
Supplier: EDVOTEK
Description: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
UOM: 1 * 1 items

MSDS


Product Image-EDVO953

Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

Catalog Number: (EDVO953)
Supplier: EDVOTEK
Description: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
UOM: 1 * 1 items
Product Image-BE-308

Experimentation kits, DNA ligation

Catalog Number: (BE-308)
Supplier: G-Biosciences
Description: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.
UOM: 1 * 1 KIT
Product Image-EDVO212

Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes

Catalog Number: (EDVO212)
Supplier: EDVOTEK
Description: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
UOM: 1 * 1 KIT
Product Image-EDVO105-C

Kits: mapping of restriction sites on plasmid DNA, Ready-to-Load™

Supplier: EDVOTEK
Description: DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Product Image-BE-405

Experimentation kits, enzyme analysis

Catalog Number: (BE-405)
Supplier: G-Biosciences
Description: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
UOM: 1 * 1 KIT
Product Image-EDVO371

Experimentation kits, DNA fingerprinting - using PCR

Catalog Number: (EDVO371)
Supplier: EDVOTEK
Description: Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.
UOM: 1 * 1 KIT

MSDS


Image Unavailable-EDVO270

Experimentation kits, antigen-antibody interaction: the ouchterlony procedure

Catalog Number: (EDVO270)
Supplier: EDVOTEK
Description: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.
UOM: 1 * 1 items
Product Image-EDVOS-48

Ready-to-Load™ Kits, what is PCR and how does it work?

Catalog Number: (EDVOS-48)
Supplier: EDVOTEK
Description: This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.
UOM: 1 * 1 KIT
Product Image-EDVO302

Experimentation kits, purification of the restriction enzyme Eco RI

Catalog Number: (EDVO302)
Supplier: EDVOTEK
Description: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
UOM: 1 * 1 KIT
Product Image-EDVO101

Kits: principles and practice of agarose gel electrophoresis, Ready-to-Load™

Supplier: EDVOTEK
Description: Ready-to-Load™ kit to show how electrophoresis separates molecules on the basis of size and charge.

Product Image-EDVO986

Experimentation kits, analysis and comparision of mammalian cell types

Catalog Number: (EDVO986)
Supplier: EDVOTEK
Description: Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
UOM: 1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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