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ADAM17 fluorometric drug discovery kit

Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
Catalog Number: ENZOBMLAK3100001
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES
Product Image-70043.

Fluorescent nerve terminal probes and stains

Description: Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.
Catalog Number: 70043.
UOM: 1 * 5 items
Supplier: Biotium
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Live bacterial gram stain kit

Description: Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.
Catalog Number: 32000-1
UOM: 1 * 1 KIT
Supplier: Biotium
Product Image-MLDVR8346

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Description: EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
Catalog Number: MLDVR8346
UOM: 1 * 1 KIT
Supplier: Molecular Devices
Image Unavailable-ENZOBMLAK5160001

FLUOR DE LYS® HDAC6 fluorometric drug discovery kit

Description: A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
Catalog Number: ENZOBMLAK5160001
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES
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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Description: A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
Catalog Number: ENZOBMLAK5180001
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES
Product Image-ENZOBMLUW09700001

Auto-Ubiquitinylation Kit

Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
Catalog Number: ENZOBMLUW09700001
UOM: 1 * 10 Tests
Supplier: ENZO LIFE SCIENCES
Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
Catalog Number: ENZOBMLUW99150001
UOM: 1 * 20 Tests
Supplier: ENZO LIFE SCIENCES
Product Image-TNB-1022-L160

Foxp3/transcription factor staining buffer

Description: Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.
Catalog Number: TNB-1022-L160
UOM: 1 * 160 mL
Supplier: Tonbo Biosciences
Product Image-2400102.

Cell Viability Reagent Kits for Beckman Coulter Vi-CELL® systems

Description: The Cell Viability Reagent Kits are designed specifically for use with the Beckman Coulter Vi-CELL® Cell Viability Analyser. The Cell Viability Reagent Kits, as well as being cost-efficient, are proven to be of equivalent quality and performance when compared to Beckman Coulter Vi-CELL® Kits.
Catalog Number: 2400102.
UOM: 1 * 200 items
Supplier: CELL VIABILITY

MSDS Certificates


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BML-280 ≥98% (by TLC)

Description: Potent and selective inhibitor of phospholipase D2 (PLD2) in cellular (PLD1 IC50 = 1900nM Calu-1 cells; PLD2 IC50 = 90nM HEK293-gfpPLD2 cells) and in vitro (hPLD1 IC50 = >20’000nM; hPLD2 IC50 = 500nM) assays.
Catalog Number: ENZOBMLST3710005
UOM: 1 * 5 mg
Supplier: ENZO LIFE SCIENCES
Image Unavailable-ENZOBMLKI1040050

Fluor-de-Lys® Deacetylase Substrate

Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
Catalog Number: ENZOBMLKI1040050
UOM: 1 * 50 µl
Supplier: ENZO LIFE SCIENCES
Product Image-ENZOALX270445M005

RepSox ≥97% (by HPLC)

Description: Cell permeable, potent, selective and ATP-competitive inhibitor of TGF-β RI kinase (IC50=23nM, 4nM and 18nM for binding, auto-phosphorylation and cellular assay in HepG2 cells of TGF-β RI kinase, respectively). Minimally affects a panel of 9 closely related kinases including p38 MAPK (IC50>16µM).
Catalog Number: ENZOALX270445M005
UOM: 1 * 5 mg
Supplier: ENZO LIFE SCIENCES
Product Image-AATB22906

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Description: Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).
Catalog Number: AATB22906
UOM: 1 * 200 Tests
Supplier: AAT BIOQUEST
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4-[(Dipropylamino)sulphonyl]benzoic acid sodium salt, water soluble

Description: Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes. The use of probenecid to block the efflux of intracellular dyes was first described by Di Virgilio et al. (1990), and it has been used with a wide range of anionic dyes and conjugates.
Catalog Number: 50027.
UOM: 1 * 1 KIT
Supplier: Biotium
Product Image-AATB21621

PhosphoWorks™ Luminometric ATP Assay Kit, Bright Glow

Description: Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling.
Catalog Number: AATB21621
UOM: 1 * 10 items
Supplier: AAT BIOQUEST
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