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Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Product Image-MILF2504

Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Catalog Number: (MILF2504)
Supplier: Merck
Description: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
UOM: 1 * 50 mL
Product Image-MANA920082

Colorimetric rapid tests, phosphate, VISOCOLOR®

Supplier: MACHEREY-NAGEL
Description: Reagents for the determination of phosphate in water samples.

MSDS

Product Image-732-3799

Brilliant III SYBR® MM, Agilent Technologies

Supplier: AGILENT
Description: Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.

Product Image-PIER89880

Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

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Hardness tablets

Supplier: Lovibond
Description: The speed test is based on reverse titration. After adding a reagent tablet to a calibrated test tube, the water sample is added slowly until the colour of the solution changes (e.g. from red to blue). The user can then obtain the result from the liquid level.

Certificates

Image Unavailable-AGLS600357

Cloned Pfu DNA polymerase alternative detergents

Supplier: AGILENT
Description: Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Image Unavailable-ENZOENZNUC1010200

AMPIGENE® HS Taq Mix

Supplier: ENZO LIFE SCIENCES
Description: Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Product Image-BIOONOVA-5150-02

NEXTFLEX™ Cell Free DNA-Sequence Kits

Supplier: BIOO SCIENTIFIC
Description: The NEXTFLEX™ cell free DNA-sequence kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, in two hours.

New Product

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PerfeCTa® qPCR FastMix™ reaction mixes

Supplier: Quantabio
Description: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

MSDS

Product Image-BIOONOVA-512962

NEXTFLEX™ RiboNaut rRNA (HMR) Depletion Kits

Supplier: BIOO SCIENTIFIC
Description: The NEXTFLEX® RiboNaut™ rRNA depletion kit (HMR) effectively removes rRNAs from mammalian total RNA while enabling labs to find additional RNA species in a sample, not only limited to intact mRNAs.

New Product

Product Image-LOVI146450

Test kits, alkalinity (discs), CHECKIT®

Catalog Number: (LOVI146450)
Supplier: Lovibond
Description: CHECKIT® Comparator is a compact and handy colorimetric unit which is suitable for both mobile and static analysis work. Supplied with a generous number of different colour scales, it provides the basis for a comprehensive, easy-to use colorimetric analysis system.
UOM: 1 * 1 KIT
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AccuStart™ II PCR SuperMix

Supplier: Quantabio
Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

MSDS Certificates

Image Unavailable-LOVI471170

Nitrite VHR L, 500 ml

Catalog Number: (LOVI471170)
Supplier: Lovibond
Description: Easily monitor nitrite levels in closed-loop cooling systems with this liquid reagent. Proper control of nitrite levels prevents pipework corrosion. This easy-to-use colourimetric method test can monitor high levels (up to 2,500 mg/L) of nitrite without the need to dilute samples.
UOM: 1 * 500 mL

New Product


Product Image-PIER89881

Cell surface protein isolation kit, Pierce™

Catalog Number: (PIER89881)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
UOM: 1 * 1 KIT
Product Image-HACHLCK242

Test kits, photometric, vicinal diketones, LCK cuvette tests

Catalog Number: (HACHLCK242)
Supplier: Hach
Description: This ready-to-use, high-precision pre-dosed cuvette kit tests for the presence of vicinal diketones (VDK) with less hassle and no chemical waste. It also simplifies brewing analysis, with accurate results, every time.
UOM: 1 * 1 KIT
Inquire for Price
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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