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Product Image-786-471

Hybridisation buffers

Supplier: G-Biosciences
Description: The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Image Unavailable-335940-10G

Xylene cyanole FF ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Image Unavailable-ROCKMB-045

SSC buffer, 20X concentrate, pH 7,0

Catalog Number: (ROCKMB-045)
Supplier: Rockland Immunochemicals
Description: Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, <i>in situ</i> hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
UOM: 1 * 1 L
Product Image-41011.

GelRed® Prestain Plus 6X DNA loading dye

Catalog Number: (41011.)
Supplier: Biotium
Description: 6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.
UOM: 1 * 1 mL

Environmentally Preferable


Image Unavailable-22011.

Blocking buffer, fish gelatin

Supplier: Biotium
Description: Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

Product Image-M124-250ML

Urea 8 mol/l in aqueous solution, Proteomics Grade

Catalog Number: (M124-250ML)
Supplier: VWR Chemicals
Description: A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.
UOM: 1 * 250 mL

MSDS Certificates


Product Image-E413-250G

MOPS-Na ≥99%, high purity

Catalog Number: (E413-250G)
Supplier: VWR Chemicals
Description: A zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA.
UOM: 1 * 250 g

MSDS Certificates


Product Image-NLO-03

Lysates, marsupial GenLysate™, opossum

Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Product Image-PL-02

Lysates, yeast GenLysate™

Catalog Number: (PL-02)
Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 150 µG
Product Image-700-0292

VWR® shiroGEL Modular mini vertical PAGE systems

Catalog Number: (700-0292)
Supplier: VWR Collection
Description: The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.
UOM: 1 * 1 items
Image Unavailable-APOSBI4545-25G

2-Amino-2-methyl-1,3-propanediol 99+%, high purity

Supplier: Apollo Scientific
Description: Buffer component used in SDS - gradient gel electrophoresis. Biological buffer - useful range 7.8-9.7.

Product Image-TLH-09

Lysates, mammalian GenLysate™, human

Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Product Image-NLR-01

Lysates, mammalian GenLysate™, rat

Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Product Image-NLC-08

Lysates, bird GenLysate™, chicken

Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Product Image-ML-04

Lysates, frog GenLysate™

Catalog Number: (ML-04)
Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 150 µG
Product Image-ML-02

Lysates, fish GenLysate™, trout

Catalog Number: (ML-02)
Supplier: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 150 µG
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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