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ReadyPour™ Luria Broth (LB) agar base

Description: Educational Materials, Microbiology, Application: Microbiology
Catalog Number: EDVO615
UOM: 1 * 1 mL
Supplier: EDVOTEK
Image Unavailable-EDVO616

ReadyPour™ Luria Broth (LB) agar with ampicillin

Description: Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin
Catalog Number: EDVO616
UOM: 1 * 170 mL
Supplier: EDVOTEK
Product Image-EDVO221

Transformation of E. coli with pGAL™

Description: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.
Catalog Number: EDVO221
UOM: 1 * 1 items
Supplier: EDVOTEK
Product Image-EDVO223/AP08

Transformation with green fluorescent protein

Description: In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish <i>Aequorea victoria</i>. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
Catalog Number: EDVO223/AP08
UOM: 1 * 1 items
Supplier: EDVOTEK
Product Image-BE-301

Experimentation kits, bacterial conjugation

Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.
Catalog Number: BE-301
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
Catalog Number: EDVO255
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-BE-201

Experimentation kits, bacterial culture and growth study

Description: This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.
Catalog Number: BE-201
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-EDVO222

Transformation with blue and green fluorescent proteins

Description: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
Catalog Number: EDVO222
UOM: 1 * 1 items
Supplier: EDVOTEK

MSDS


Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
Catalog Number: BE-203
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-BE-202

Experimentation kits, bacterial gram staining

Description: The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.
Catalog Number: BE-202
UOM: 1 * 1 KIT
Supplier: G-Biosciences

Certificates


Product Image-RDBT002015

VWR® Electrode Holders

Description: Plain borosilicate tubes for insertion of a wire electrode so that the electrode is insulated from the clamp or holder and protected from mechanical damage. The tube also helps the flexible wire electrode be positioned correctly in a rigid support.
Catalog Number: RDBT002015
UOM: 1 * 100 items
Supplier: VWR Chemicals
Product Image-BE-502

Experimentation kits, dot blot analysis

Description: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
Catalog Number: BE-502
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-201-0216

Capillary tube on foot

Description: Five communicating glass tubes with different inner diameters. Used to show capillary forces and how they are affected by the inner diameter of the pipes.
Catalog Number: 201-0216
UOM: 1 * 1 items
Supplier: GLASWARENFABRIK KARL HECHT
Product Image-765-0969

Communicating tubes

Description: Five glass tubes of different shape and inner dimensions. Down to each other. When filling the liquid in the pipes, it is clear that the height of the stowage in the tubes. pipes are independent of the design of the pipes. Comes with foot.
Catalog Number: 765-0969
UOM: 1 * 1 items
Supplier: GLASWARENFABRIK KARL HECHT
Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Description: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
Catalog Number: BE-404
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-BE-401

Experimentation kits, physical properties of proteins

Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
Catalog Number: BE-401
UOM: 1 * 1 KIT
Supplier: G-Biosciences
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