You Searched For: N-Succinimidyl+pentafluorobenzoate

Catalog Number: (573100-100)

Supplier: Merck Millipore (Calbiochem‎)

Description: A useful agent for introducing thiol groups, in protected form, into various proteins. Reacts with primary amines to add protected sulfhydryl groups. When treated with hydroxylamine, yields a free sulfhydryl group that can be conjugated to maleimide-modified proteins.

UOM: 1 * 100 mg

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Catalog Number: (AATB2021)

Supplier: AAT BIOQUEST

Description: An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

UOM: 1 * 25 mg

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Catalog Number: (PIER21857)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

UOM: 1 * 50 mg

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Catalog Number: (90022.)

Supplier: Biotium

Description: 5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

UOM: 1 * 25 mg

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Catalog Number: (PIER22360)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

UOM: 1 * 50 mg

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Catalog Number: (90032.)

Supplier: Biotium

Description: Amine-reactive form of 5-carboxyrhodamine 6G single isomer.

UOM: 1 * 5 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Catalog Number: (AATB475)

Supplier: AAT BIOQUEST

Description: The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen).

UOM: 1 * 5 mg

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Catalog Number: (AATB1050)

Supplier: AAT BIOQUEST

Description: iFluor® 597 is a unique color for fluorescence imaging and flow cytometry applications.

UOM: 1 * 1 mg

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Catalog Number: (AATB1371)

Supplier: AAT BIOQUEST

Description: iFluor® 780 dyes have fluorescence excitation and emission maxima of ~784 nm and ~808 nm respectively.

UOM: 1 * 1 mg

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Catalog Number: (AATB1034)

Supplier: AAT BIOQUEST

Description: iFluor® 570 family has fluorescence that is pH-independent from pH 3 to 11.

UOM: 1 * 1 mg

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Catalog Number: (AATB1043)

Supplier: AAT BIOQUEST

Description: iFluor® 675 dyes have fluorescence excitation and emission maxima of ~677 nm and ~710 nm respectively.

UOM: 1 * 1 mg

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Catalog Number: (90089.)

Supplier: Biotium

Description: 5-ROX-PEO8 SE (full name: 5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group.

UOM: 1 * 1 mg

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Catalog Number: (AATB1046)

Supplier: AAT BIOQUEST

Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

UOM: 1 * 1 mg

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Catalog Number: (AATB1047)

Supplier: AAT BIOQUEST

Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

UOM: 1 * 1 mg

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Catalog Number: (AATB39060)

Supplier: AAT BIOQUEST

Description: Psoralens and their derivatives (such as 8-TMP and 4,5’,8-TMP) are well known to have unique crosslinking features to DNA.

UOM: 1 * 5 mg

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