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ExoBrite™ True EV Membrane Stains

Supplier: Biotium
Description: Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium
Description: Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Phosphoserine detection set

Catalog Number: (ENZOALX850023KI01)
Supplier: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET
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Antibody labelling kits, Mix-n-Stain™ FITC

Supplier: Biotium
Description: Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

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Gold 550 dUTP

Catalog Number: (ENZOENZ42521)
Supplier: ENZO LIFE SCIENCES
Description: Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for <i>E.coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
UOM: 1 * 25 Nmo
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Reactive oxygen species indicators dyes

Supplier: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

MSDS

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Bio-16-ddUTP, tetralithium salt

Catalog Number: (ENZOENZ42813)
Supplier: ENZO LIFE SCIENCES
Description: Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.
UOM: 1 * 25 Nmo
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Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium
Description: A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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NEDDylation kit

Catalog Number: (ENZOBMLUW05900001)
Supplier: ENZO LIFE SCIENCES
Description: This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.
UOM: 1 * 20 Tests
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ZnAF-2.tetrahydrochloride

Catalog Number: (ENZOALX620072M001)
Supplier: ENZO LIFE SCIENCES
Description: Fluorescent probe for Zn2+
UOM: 1 * 1 mg
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SeramunBlock

Supplier: Apollo Scientific
Description: Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

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femtoCHROMO™- AP Substrate for chromogenic detection

Supplier: G-Biosciences
Description: A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

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Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Supplier: Biotium
Description: Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

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CF™ dye bovine serum albumin conjugates

Supplier: Biotium
Description: Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.

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Tyramide amplification kit with HRP goat, anti-mouse and CF® dye or biotin tyramide

Supplier: Biotium
Description: Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Nuclear-ID™ Blue/Green cell viability reagent

Catalog Number: (ENZOENZ53004C100)
Supplier: ENZO LIFE SCIENCES
Description: Nucleus dye
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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