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Product Image-786-411

Protein extraction kit, Insect PE LB™

Catalog Number: (786-411)
Supplier: G-Biosciences
Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL
Image Unavailable-ENZOADI801526

Extraction reagents

Supplier: ENZO LIFE SCIENCES
Description: Concentrated lysis buffer for preparation of cell and tissue extracts.

Product Image-733-2877

VWR®, Fast extract DNA solution

Supplier: VWR Chemicals
Description: The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.

Product Image-786-180

Protein extraction kit, mammalian cell PE LB™

Catalog Number: (786-180)
Supplier: G-Biosciences
Description: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
UOM: 1 * 500 mL
Product Image-TB110

Kingdom blot

Catalog Number: (TB110)
Supplier: G-Biosciences
Description: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM: 1 * 1 items
Product Image-733-2879

VWR® Fast extract genotyping PCR kit

Supplier: VWR Chemicals
Description: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Product Image-G193-05

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor
Description: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Product Image-PIER78870

Pierce™ DNA Extraction Reagent Kit, Yeast

Catalog Number: (PIER78870)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ yeast DNA extraction reagent kit uses Y-PER yeast protein extraction reagent to quickly extract and purify genomic and plasmid DNA from yeast.
UOM: 1 * 1 KIT
Product Image-PIER20395

Immobilised jacalin agarose, Pierce™

Catalog Number: (PIER20395)
Supplier: Thermo Fisher Scientific
Description: Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.
UOM: 1 * 5 mL

MSDS


Product Image-TNB-4300-L100

RBC Lysis Buffer (10X)

Catalog Number: (TNB-4300-L100)
Supplier: Tonbo Biosciences
Description: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
UOM: 1 * 100 mL

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Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Catalog Number: (786-316)
Supplier: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLSW1010200

Cell extract HL60 (non-induced)

Catalog Number: (ENZOBMLSW1010200)
Supplier: ENZO LIFE SCIENCES
Description: Whole-cell extract of human HL-60 leukemia cells.
UOM: 1 * 200 µl
Image Unavailable-ICNA04818048

Phenol, white solid, Ultrapure

Catalog Number: (ICNA04818048)
Supplier: MP Biomedicals
Description: Phenol is useful for extraction of viral, plasmid and phage DNAs, and to solubilise and dissociate proteins. It is used as a general disinfectant, either in solution or mixed with slaked lime, etc., for toilets, stables, cesspools, floors, drains, etc.; for the manufacture of colorless or light-colored artificial resins, many medical and industrial organic compounds and dyes; as a reagent in chemical analysis. Aqueous solutions are used as topical anesthetic; topical antiseptic; topical antipruritic. Phenol is used in DNA extractions for removing protein.
UOM: 1 * 500 g
Product Image-786-033

Research buffers and reagents

Supplier: G-Biosciences
Description: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Product Image-PIER75705

β-Galactosidase Assay Kit and Reagents

Supplier: Thermo Fisher Scientific
Description: The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Product Image-786-132

GET™ Total RNA kit

Catalog Number: (786-132)
Supplier: G-Biosciences
Description: The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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