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Product Image-UFC903096

Centrifugal filter units, Amicon® Ultra-15

Supplier: Merck
Description: These centrifugal filter units can be used for a number of applications: concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms; purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS; concentration of dilute or pre-purified proteins from column eluents; desalting, buffer exchange and protein dialysis; size exclusion and protein fractionation.

Product Image-786-271

Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Catalog Number: (786-271)
Supplier: G-Biosciences
Description: SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.
UOM: 1 * 1 KIT
Image Unavailable-BNC940232-500

Anti-Phosphotyrosine Mouse Monoclonal Antibody (CF594) [clone: PY20]

Supplier: Biotium
Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Image Unavailable-BNC880793-500

Anti-Phosphotyrosine Mouse Monoclonal Antibody (CF488A) [clone: PY793]

Supplier: Biotium
Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Image Unavailable-BNC400793-500

Anti-Phosphotyrosine Mouse Monoclonal Antibody (CF640R) [clone: PY793]

Supplier: Biotium
Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Image Unavailable-BOSSBS-0362R-CY5

Anti-Protein A Rabbit Polyclonal Antibody (Cy5®)

Catalog Number: (BOSSBS-0362R-CY5)
Supplier: Bioss
Description: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
UOM: 1 * 100 µl
Product Image-BSENPE-019-100

Mouse native NGF (from submaxillary glands)

Supplier: Biosensis
Description: Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF.

Certificates

Product Image-PIER20340

Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel

Supplier: Thermo Fisher Scientific
Description: Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Image Unavailable-ENZOBMLKI1040050

Fluor-de-Lys® Deacetylase Substrate

Catalog Number: (ENZOBMLKI1040050)
Supplier: ENZO LIFE SCIENCES
Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
UOM: 1 * 50 µl
Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests
Product Image-PIER45212

Melon™ Gel IgG Purification Kit

Catalog Number: (PIER45212)
Supplier: Thermo Fisher Scientific
Description: The Melon Gel IgG purification kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.
UOM: 1 * 1 KIT
Image Unavailable-BOSSBS-0966R

Anti-HA tag Rabbit Polyclonal Antibody

Catalog Number: (BOSSBS-0966R)
Supplier: Bioss
Description: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
UOM: 1 * 100 µl
Image Unavailable-ENZOBMLEI3410001

Geldanamycin ≥98% Biotin

Catalog Number: (ENZOBMLEI3410001)
Supplier: ENZO LIFE SCIENCES
Description: A useful tool for affinity purification of HSP-90 and HSP-90 dependent client proteins.
UOM: 1 * 1 mg
Product Image-PIER20399

Protein G agarose, Pierce™

Supplier: Thermo Fisher Scientific
Description: Pierce™ Protein G agarose is a premium quality affinity resin for antibody purification.

MSDS

Product Image-PIER77676

Recombinant protein G, Pierce™

Supplier: Thermo Fisher Scientific
Description: Purified (unconjugated) Pierce™ Recombinant Protein G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Image Unavailable-NOVUNBP2-31055F

Anti-His-tag Mouse Monoclonal Antibody [clone: HIS.H8] (FITC (Fluorescein Isothiocyanate))

Catalog Number: (NOVUNBP2-31055F)
Supplier: Novus Biologicals
Description: A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection.
UOM: 1 * 0,1 mL

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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