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Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Product Image-PIER89821

Complete bacterial protein extraction reagent, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Accessories for protein kits

Supplier: G-Biosciences
Description: EZ-Grind™ grinding resin for the extraction of both proteins and DNA

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Silver Protein

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.

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Protein extraction reagents, CytoBuster™

Supplier: Merck Millipore (Novagen)
Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Product Image-786-225

Total protein extraction kit, TPE™

Catalog Number: (786-225)
Supplier: G-Biosciences
Description: Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
UOM: 1 * 1 KIT
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EpiXtract® total histone extraction kit

Catalog Number: (ENZOENZ450140100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
UOM: 1 * 1 KIT
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Protein extraction kits, ProteoExtract™

Supplier: Merck Millipore (Calbiochem‎)
Description: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

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EpiXtract® total histone extraction kit (high throughput)

Catalog Number: (ENZOENZ450130002)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
UOM: 1 * 1 KIT
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Protein extraction kits, BugBuster® Plus Lysonase™

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

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Membrane protein extraction kit, Pierce™ Mem-PER™ Plus

Catalog Number: (PIER89842)
Supplier: Thermo Fisher Scientific
Description: The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
UOM: 1 * 1 KIT

MSDS


Product Image-786-182

Protein fractionation kit, nuclear and cytoplasmic extraction kit

Catalog Number: (786-182)
Supplier: G-Biosciences
Description: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
UOM: 1 * 1 KIT
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Malt extract

Catalog Number: (OXOILP0039B)
Supplier: THERMO FISHER DIAGNOSTICS
Description: Malt extract
UOM: 1 * 500 g

MSDS Certificates


Product Image-786-077

Protein reducing agents

Supplier: G-Biosciences
Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

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Accessories for primary cell isolation kits, Pierce™

Supplier: Thermo Fisher Scientific
Description: Neuronal Protein Extraction Reagent (N-PER), sufficient reagent to extract protein from ~10 g of cells or tissue, Pierce

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Protein fractionation kit, FOCUS™ Membrane Proteins

Catalog Number: (786-249)
Supplier: G-Biosciences
Description: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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