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Fluor-de-Lys® Developer I concentrate

Catalog Number: (ENZOBMLKI1050300)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl
Image Unavailable-AGLS214303

Affinity protein expression and purification systems

Catalog Number: (AGLS214303)
Supplier: AGILENT
Description: The affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification.
UOM: 1 * 10 mL
Product Image-786-803

Antibody clean up kit, Pearl™

Catalog Number: (786-803)
Supplier: G-Biosciences
Description: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.
UOM: 1 * 1 KIT
Product Image-786-218

Concanavalin A agarose

Supplier: G-Biosciences
Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Image Unavailable-ENZOBMLSE5280010

Human recombinant DPP9

Catalog Number: (ENZOBMLSE5280010)
Supplier: ENZO LIFE SCIENCES
Description: Produced in insect cells. Active recombinant human DPP9 with an N-terminal purification tag.
UOM: 1 * 10 µG
Product Image-AGLS5185-5988

Multiple affinity removal starter reagent kits

Supplier: AGILENT
Description: Starter reagent kit for multiple affinity removal LC columns.

Product Image-8183-04

J.T.Baker® BAKERBOND®, Supported Liquid Extraction Plates

Supplier: Avantor
Description: J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

Image Unavailable-AGLS240214

InterPlay Adenoviral TAP Systems

Supplier: AGILENT
Description: The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

Product Image-PIER78248

Bacterial Protein Extraction Kits, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Product Image-731-0711

KingFisher™ Apex purification system

Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific KingFisher Apex represents the pinnacle of automated purification for DNA, RNA, proteins, and cells. Building on decades of KingFisher product value and excellence, we’ve combined superior instrument capabilities with complete touch screen-based control to deliver unrivalled flexibility and performance. The KingFisher Apex makes sample prep simple.

Image Unavailable-ENZOBMLSE5270010

Human recombinant DPP8

Catalog Number: (ENZOBMLSE5270010)
Supplier: ENZO LIFE SCIENCES
Description: Produced in insect cells. Active recombinant human DPP8 with an N-terminal purification tag.
UOM: 1 * 10 µG
Product Image-786-598

Biotin resin

Catalog Number: (786-598)
Supplier: G-Biosciences
Description: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM:


Product Image-PIER28665

Iodination beads, Pierce™

Catalog Number: (PIER28665)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Iodination beads are Ø 3 mm polystyrene beads that are coated with an oxidising reagent, which provides efficient activation of <sup>125</sup>I for protein or peptide iodination procedures.
UOM: 1 * 50 items
Product Image-786-552

Calmodulin Resin Kit for CBP purification

Supplier: G-Biosciences
Description: The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

Product Image-786-724

Disposable spin columns for protein purification

Supplier: G-Biosciences
Description: These disposable spin columns are designed for protein purification. These spin columns are uniquely designed, with a snap off end converting to a closure for the column for easy manipulation and use.

Image Unavailable-ENZOBMLSE5450010

Human recombinant POP/PREP

Catalog Number: (ENZOBMLSE5450010)
Supplier: ENZO LIFE SCIENCES
Description: Produced in insect cells. Active recombinant human POP/PREP/PEP with an N-terminal purification tag.
UOM: 1 * 10 µG
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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