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Nitro blue tetrazolium chloride (NBT) for molecular biology

Supplier: MP Biomedicals
Description: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Product Image-PIER34035

Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)

Catalog Number: (PIER34035)
Supplier: Thermo Fisher Scientific
Description: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
UOM: 1 * 1 g
Image Unavailable-MOLEM13118525

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

Catalog Number: (MOLEM13118525)
Supplier: Molekula
Description: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
UOM: 1 * 1 g

Market Source Item This is a MarketSource item. Additional charges may apply

Image Unavailable-MOLE14706101-5G

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

Catalog Number: (MOLE14706101-5G)
Supplier: Molekula
Description: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
UOM: 1 * 5 g

Market Source Item This is a MarketSource item. Additional charges may apply

Image Unavailable-ICNA0219459280

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ≥98%, yellow powder cell culture reagent

Supplier: MP Biomedicals
Description: Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

MSDS

Image Unavailable-ICNA02102227.1

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ~98%, yellow powder

Supplier: MP Biomedicals
Description: 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide(MTT) is an histochemical/cytochemical reagent. It is converted to water-insoluble MTT-formazan of dark blue color by mitochondrial dehydrogenases of living cells, thus used for measurement of cell division.

Image Unavailable-PIER34040

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate) substrate ≥99%

Catalog Number: (PIER34040)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
UOM: 1 * 1 g
Image Unavailable-475989-1

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Sigma-Aldrich®

Supplier: Merck Millipore (Calbiochem‎)
Description: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Sigma-Aldrich®

Image Unavailable-A16008.03

Tetrazolium violet ≥98%

Supplier: Thermo Fisher Scientific
Description: Tetrazolium violet ≥98%

MSDS Certificates

Image Unavailable-APOSBIC1455-50MG

5-Cyano-2,3-ditolyl tetrazolium chloride

Catalog Number: (APOSBIC1455-50MG)
Supplier: Apollo Scientific
Description: Tetrazolium dye which produces a fluorescent formazandye in coupled redox reactions
UOM: 1 * 50 mg
Image Unavailable-T8877-10G

2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: 2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Product Image-28804.121

2,3,5-Triphenyltetrazolium chloride (TTC), TECHNICAL

Catalog Number: (28804.121)
Supplier: VWR Chemicals
Description: 2,3,5-Triphenyltetrazolium chloride (TTC), TECHNICAL
UOM: 1 * 10 g

MSDS Certificates


Image Unavailable-I8377-25G

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride colorimetric assays, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Image Unavailable-M8640-500MG

1-Methoxy-5-methylphenazinium methyl sulphate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: 1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

Product Image-N812-100MG

XTT sodium salt, high purity

Catalog Number: (N812-100MG)
Supplier: VWR Chemicals
Description: XTT sodium salt, high purity
UOM: 1 * 100 mg

MSDS


Image Unavailable-A6246.0025

2,3,5-Triphenyltetrazolium chloride (TTC)

Catalog Number: (A6246.0025)
Supplier: PanReac AppliChem
Description: 2,3,5-Triphenyltetrazolium chloride (TTC)
UOM: 1 * 25 g

Certificates


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
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